In this guide, I will show you how to perform the delta-delta Ct method for calculating relative gene expression values in qPCR by using Microsoft Excel.

With this data, you will work in groups and proceed to calculate the change in expression values for each gene in liver and brain tissue. The CT data is used to determine the amount of each …

The 2-ΔΔCT method has been extensively used as a relative quantification strategy for quantitative real-time polymerase chain reaction (qPCR) data analysis. This method is a …

Delta-Delta Ct method or Livak method is the most preferred method for qPCR data analysis. However, it can only be used when certain criteria are met. Please refer the lecture notes to …

Sep 13, 2025 · The Delta-Delta Ct (ΔΔCt) method is a widely used technique for relative quantification in qPCR. It measures the change in expression of a target gene relative to a …

Mar 24, 2025 · The delta-delta Ct method is a common approach for relative quantification of gene expression. It involves normalizing the target gene expression to a reference gene and …

The ΔΔCT method, also known as the Livak method, is a widely accepted approach for relative quantification of gene expression using qPCR data. It compares the CT (Cycle Threshold) …

Apr 29, 2025 · The Comparative CT method, also known as the ΔΔCT method, is a widely adopted approach in real-time PCR for quantifying relative gene expression. It relies on …

Jul 12, 2025 · The ddCt (delta-delta Ct) method is a mathematical model used in real-time quantitative Polymerase Chain Reaction (qPCR) to determine the relative fold change in target …

Jul 10, 2024 · Step 1: Perform qPCR and collect Ct values for target and reference genes. Step 2: Calculate ΔCt for each sample (ΔCt = Ct (Target) - Ct (Reference)). Step 3: Calculate ΔΔCt …